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Proteomic study of non-typable Haemophilus influenzae.

Journal article
Authors Kajsa Thorén
Elisabet Gustafsson
Annica Clevnert
Thomas Larsson
Jörgen Bergström
Carol L Nilsson
Published in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Volume 782
Issue 1-2
Pages 219-26
ISSN 1570-0232
Publication year 2002
Published at Institute of Medical Biochemistry
Pages 219-26
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Bacterial Proteins, chemistry, Electrophoresis, Gel, Two-Dimensional, Haemophilus influenzae, chemistry, Mass Spectrometry, Proteomics
Subject categories Chemistry

Abstract

Non-typable Haemophilus influenzae (NTHi) are small, gram-negative bacteria and are strictly human pathogens, causing acute otitis media, sinusitis and community-acquired pneumonia. There is no vaccine available for NTHi, as there is for H. influenzae type b. Recent advances in proteomic techniques are finding novel applications in the field of vaccinology. There are several protein separation techniques available today, each with inherent advantages and disadvantages. We employed a combined proteomics approach, including sequential extraction and analytical two-dimensional polyacrylamide electrophoresis (2D PAGE), and two-dimensional semi-preparative electrophoresis (2D PE), in order to study protein expression in the A4 NTHi strain. Although putative vaccine candidates were identified with both techniques, 11 of 15 proteins identified using the 2D PE approach were not identified by 2D PAGE, demonstrating the complementarily of the two methods.

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