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Mutation profiling of adenoid cystic carcinomas from multiple anatomical sites identifies mutations in the RAS pathway, but no KIT mutations

Journal article
Authors Daniel Wetterskog
P. M. Wilkerson
D. N. Rodrigues
M. B. Lambros
K. Fritchie
Mattias K Andersson
R. Natrajan
A. Gauthier
S. Di Palma
S. Shousha
Z. Gatalica
C. Topfer
V. Vukovic
R. A'Hern
B. Weigelt
A. Vincent-Salomon
Göran Stenman
B. P. Rubin
J. S. Reis
Published in Histopathology
Volume 62
Issue 4
Pages 543-550
ISSN 0309-0167
Publication year 2013
Published at Institute of Biomedicine, Department of Pathology
Sahlgrenska Cancer Center
Pages 543-550
Language en
Links dx.doi.org/10.1111/his.12050
Keywords adenoid cystic carcinoma; BRAF ; HRAS ; KIT ; sequencing
Subject categories Pathology

Abstract

Aims The majority of adenoid cystic carcinomas (AdCCs), regardless of anatomical site, harbour the MYB–NFIB fusion gene. The aim of this study was to characterize the repertoire of somatic genetic events affecting known cancer genes in AdCCs. Methods and results DNA was extracted from 13 microdissected breast AdCCs, and subjected to a mutation survey using the Sequenom OncoCarta Panel v1.0. Genes found to be mutated in any of the breast AdCCs and genes related to the same canonical molecular pathways, as well as KIT, a proto-oncogene whose protein product is expressed in AdCCs, were sequenced in an additional 68 AdCCs from various anatomical sites by Sanger sequencing. Using the Sequenom MassARRAY platform and Sanger sequencing, mutations in BRAF and HRAS were identified in three and one cases, respectively (breast, and head and neck). KIT, which has previously been reported to be mutated in AdCCs, was also investigated, but no mutations were identified. Conclusions Our results demonstrate that mutations in genes pertaining to the canonical RAS pathway are found in a minority of AdCCs, and that activating KIT mutations are either absent or remarkably rare in these cancers, and unlikely to constitute a driver and therapeutic target for patients with AdCC.

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