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Bioinformatic strategies for unambiguous identification of prostate specific antigen in clinical samples

Journal article
Authors A Vegvari
M Rezeli
J Hakkinen
Carina Sihlbom
Elisabet Carlsohn
J Malm
H Lilja
T Laurell
G Marko-Varga
Published in JOURNAL OF PROTEOMICS
Volume 75
Issue 1
Pages 202-210
ISSN 1874-3919
Publication year 2011
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 202-210
Language en
Links dx.doi.org/10.1016/j.jprot.2011.06....
Keywords prostate specific antigen isoforms, maldi ltq orbitrap xl, esi-ltq ft-icr, proteios software environment, kallikrein-2
Subject categories Basic Medicine

Abstract

Abstract: Prostate specific antigen (PSA), as a widely used clinical biomarker in prostate cancer diagnostics, exists in multiple molecular forms. However, all of these forms might not be recognized in a given sample by the standard immunoassays. Therefore, we have investigated PSA isoforrns, separated by size, using mass spectrometric analyses. The objective of these developments was to identify and specify the various forms of PSA. To optimize successful identification of different PSA forms, we have developed a bioinformatic strategy, consisting of high resolution MALDI-MS PMF and sequencing MS/MS data searches. To improve sequence-based identification, the recently introduced Proteios software environment was employed, allowing the combination of multiple database search engines in an automated manner.

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