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Novel 3D culture system with similarities to the human heart for studies of the cardiac stem cell niche.

Journal article
Authors Marianne Jonsson
Helena Barreto Henriksson
Margret Hagman
Kristina Kajic
Anders Lindahl
Anders Jeppsson
Håkan Berggren
Julia Asp
Published in Regenerative medicine
Volume 5
Issue 5
Pages 725-36
ISSN 1746-076X
Publication year 2010
Published at Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
Institute of Medicine, Department of Emergeny and Cardiovascular Medicine
Institute of Clinical Sciences, Department of Orthopaedics
Pages 725-36
Language en
Links dx.doi.org/10.2217/rme.10.63
Subject categories Cell biology

Abstract

AIMS: The aim of this study was to develop a 3D culture system with similarities to the human heart, which was suitable for studies of adult cardiac stem or progenitor cells. MATERIALS & METHODS: Dissociated cells from human cardiac biopsies were placed in high-density pellet cultures and cultured for up to 6 weeks. Gene and protein expressions, analyzed by quantitative real-time PCR and immunohistochemistry, and morphology were studied in early and late pellets. RESULTS: Cells cultured in the 3D model showed similarities to human cardiac tissue. Moreover, markers for cardiac stem and progenitor cells were also detected after 6 weeks of culture, in addition to markers for signaling pathways active in stem cell niche regulation. CONCLUSIONS: The described 3D culture model could be a valuable tool when studying the influence of different compounds on proliferation and differentiation processes in cardiac stem or progenitor cells in cardiac regenerative research.

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