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A study of the expression of Cyclin E and its isoforms in tumor and adjacent mucosa, correlated to patient outcome in early colon cancer.

Journal article
Authors Irina Corin
Lars Larsson
Jörgen Bergström
Bengt Gustavsson
Kristoffer Derwinger
Published in Acta oncologica (Stockholm, Sweden)
Volume 49
Issue 1
Pages 63-69
ISSN 1651-226X
Publication year 2010
Published at Institute of Clinical Sciences, Department of Surgery
Core Facilities, Proteomics
Pages 63-69
Language en
Links dx.doi.org/10.3109/0284186090326804...
Keywords Aged, Aged, 80 and over, Blotting, Western, Colonic Neoplasms, metabolism, mortality, pathology, Cyclin E, biosynthesis, Female, Humans, Intestinal Mucosa, metabolism, Male, Middle Aged, Neoplasm Recurrence, Local, metabolism, Neoplasm Staging, Prognosis, Protein Isoforms, biosynthesis, Tumor Markers, Biological, analysis
Subject categories Cancer and Oncology

Abstract

BACKGROUND: Cyclin E, a key regulator in the cell cycle, is often over-expressed in malignant disease. It can present as full length (FL) and low-molecular-weight (LMW) isoforms. The purpose of this study was to characterize the expression pattern of cyclin E in colon cancer, both in tumor and in macroscopically normal adjacent mucosa. A secondary aim was to study the possible correlation to clinical factors and patient outcome. MATERIAL AND METHOD: Tumor and mucosa tissue from 114 patients with radically operated, non-metastatic colon tumors were analyzed. The cyclin E expression was measured by Western Blot in the tumor and adjacent mucosa using the antibody targeting C-terminal. The cyclin E expression was correlated to both pathology factors as differentiation grade and to the patient outcome. RESULTS: Cyclin E was detected in both tumor and adjacent mucosa and in both FL and LMW-forms. FL was present in 29 (25.4%) tumors and only in three (2.6%) mucosa samples, the corresponding figures for the LMW-isoforms were 80 (70.2%) and 67 (58.8%). There was no correlation between the cyclin E expression and gender, age, tumor location or tumor pathology. Patients with a high expression of LMW isoforms (p < 0.03) or a high total expression (FL+LMW) (p < 0.006) had higher risks of recurrence and thus a worse survival. CONCLUSION: Cyclin E is expressed in FL- and LMW-forms in both colon tumors and the macroscopically normal adjacent mucosa. A high expression of cyclin E in tumor was associated with an increased risk of tumor recurrence and a worse outcome. It could be a possible prognostic marker in non-metastatic colon cancer.

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