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Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins

Journal article
Authors János T Padra
Q. Pagneux
J. Bouckaert
R. Jijie
Henrik Sundh
R. Boukherroub
S. Szunerits
Sara K. Lindén
Published in Biosensors and Bioelectronics
Volume 146
ISSN 0956-5663
Publication year 2019
Published at SWEMARC
Department of Biological and Environmental Sciences
Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Language en
Links dx.doi.org/10.1016/j.bios.2019.1117...
Subject categories Cell Biology

Abstract

Knowledge on host-pathogen interactions contributes to the development of approaches to alleviate infectious disease. In this work, we developed a surface plasmon resonance (SPR) based method for investigating bacteria/mucins interactions. Furthermore, we investigated adhesion of three pathogens, Aeromonas salmonicida, Aeromonas hydrophila and Vibrio harveyi, to Atlantic salmon mucins isolated from different epithelial sites, using SPR and microtiter-based binding assays. We demonstrated that performing bacterial binding assays to mucins using SPR is feasible and has advantages over microtiter-based binding assays, especially under flow conditions. The fluid flow in the SPR is linear and continuous and SPR enables real-time reading of mucin-bacterial bonds, which provides an in vivo-like setup for analysis of bacterial binding to mucins. The variation between technical replicates was smaller using SPR detection compared to the adenosine 5′-triphosphate (ATP) bioluminescence assay in microtiter plates. Furthermore, we demonstrated that the effect of flow on pathogen-mucin interaction is significant and that bacterial adhesion differ non-linearly with flow rates and depend on the epithelial source of the mucin. © 2019 Elsevier B.V.

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Denna text är utskriven från följande webbsida:
http://www.gu.se/english/research/publication/?publicationId=287620
Utskriftsdatum: 2020-02-26