To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Induction of mucosal immu… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Induction of mucosal immune responses against Helicobacter pylori infection after sublingual and intragastric route of immunization

Journal article
Authors Louise Sjökvist Ottsjö
Frida Jeverstam
Linda Yrlid
Ulf Alexander Wenzel
A. K. Walduck
Sukanya Raghavan
Published in Immunology
Volume 150
Issue 2
Pages 172-183
ISSN 0019-2805
Publication year 2017
Published at Institute of Biomedicine, Department of Microbiology and Immunology
Pages 172-183
Language en
Links doi.org/10.1111/imm.12676
Keywords cytokines, dendritic cells, mucosa, T cells, vaccination, vaccine-induced protection, dendritic cells, cholera-toxin, vibrio-cholerae, peyers-patches, in-vivo, adjuvant, mice, antibodies, migration, Immunology
Subject categories Immunology in the medical area, Immunology

Abstract

There is a current lack of effective mucosal vaccines against major gastroenteric pathogens and particularly against Helicobacter pylori, which causes a chronic infection that can lead to peptic ulcers and gastric cancer in a subpopulation of infected individuals. Mucosal CD4(+) T-cell responses have been shown to be essential for vaccine-induced protection against H. pylori infection. The current study addresses the influence of the adjuvant and site of mucosal immunization on early CD4(+) T-cell priming to H. pylori antigens. The vaccine formulation consisted of H. pylori lysate antigens and mucosal adjuvants, cholera toxin (CT) or a detoxified double-mutant heat-labile enterotoxin from Escherichia coli (dmLT), which were administered by either the sublingual or intragastric route. We report that in vitro, adjuvants CT and dmLT induce up-regulation of pro-inflammatory gene expression in purified dendritic cells and enhance the H. pylori-specific CD4(+) T-cell response including interleukin-17A (IL-17A), interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha) secretion. In vivo, sublingual immunization led to an increased frequency of IL-17A(+), IFN-gamma(+) and TNF-alpha(+) secreting CD4(+) T cells in the cervical lymph nodes compared with in the mesenteric lymph nodes after intragastric immunization. Subsequently, IL-17A(+) cells were visualized in the stomach of sublingually immunized and challenged mice. In summary, our results suggest that addition of an adjuvant to the vaccine clearly activated dendritic cells, which in turn, enhanced CD4(+) T-cell cytokines IL-17A, IFN-gamma and TNF-alpha responses, particularly in the cervical lymph nodes after sublingual vaccination.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?

Denna text är utskriven från följande webbsida:
http://www.gu.se/english/research/publication/?publicationId=252356
Utskriftsdatum: 2019-08-21