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Validation of a flow cytometry-based detection of γ-H2AX, to measure DNA damage for clinical applications.

Journal article
Authors Pegah Johansson
Anders Fasth
Torben Ek
Ola Hammarsten
Published in Cytometry. Part B, Clinical cytometry
Volume 92
Pages 534–540
ISSN 1552-4957
Publication year 2017
Published at Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
Institute of Clinical Sciences, Department of Pediatrics
Pages 534–540
Language en
Subject categories Pediatrics, Medical cell biology


The nucleosomal histone protein H2AX is specifically phosphorylated (γ-H2AX) adjacent to DNA double-strand breaks (DSBs) and is used for quantifying DSBs. Many chemotherapies and ionizing radiation (IR) used in cancer treatment result in DSBs. Therefore, γ-H2AX has a significant potential as a biomarker in evaluating patient sensitivity and responsiveness to IR and chemotherapy.

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Utskriftsdatum: 2020-07-16