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An inflammatory equine model demonstrates dynamic changes of immune response and cartilage matrix molecule degradation in vitro

Journal article
Authors Emilia Svala
M. Lofgren
Carina Sihlbom
Ulla Rüetschi
Anders Lindahl
S. Ekman
Eva Skiöldebrand
Published in Connective Tissue Research
Volume 56
Issue 4
Pages 315-325
ISSN 0300-8207
Publication year 2015
Published at Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
Core Facilities, Proteomics
Pages 315-325
Language en
Links dx.doi.org/10.3109/03008207.2015.10...
Keywords Horse, IL-1 beta, matrix molecules, osteoarthritis, proteomics, HUMAN SYNOVIAL-FLUID, ARTICULAR-CARTILAGE, JOINT DISEASE, PROTEIN, THROMBOSPONDIN-5, INTERGLOBULAR DOMAIN, AGGRECAN FRAGMENTS, COMPLEMENT-SYSTEM, COLLAGEN-IX, OSTEOARTHRITIS, INTERLEUKIN-1-BETA
Subject categories Clinical Medicine

Abstract

The molecular aspects of inflammation were investigated in equine articular cartilage explants using quantitative proteomics. Articular cartilage explants were stimulated with interleukin (IL)-1 beta in vitro for 25 days, and proteins released into cell culture media were chemically labeled with isobaric mass tags and analyzed by liquid chromatography-tandem mass spectrometry. A total of 127 proteins were identified and quantified in media from explants. IL-1 beta-stimulation resulted in an abundance of proteins related to inflammation, including matrix metalloproteinases, acute phase proteins, complement components and IL-6. Extracellular matrix (ECM) molecules were released at different time points, and fragmentation of aggrecan and cartilage oligomeric matrix protein was observed at days 3 and 6, similar to early-stage OA in vivo. Degradation products of the collagenous network were observed at days 18 and 22, similar to late-stage OA. This model displays a longitudinal quantification of released molecules from the ECM of articular cartilage. Identification of dynamic changes of extracellular matrix molecules in the secretome of equine explants stimulated with IL-1 beta over time may be useful for identifying components released at different time points during the spontaneous OA process.

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Denna text är utskriven från följande webbsida:
http://www.gu.se/english/research/publication/?publicationId=223002
Utskriftsdatum: 2019-08-19