To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Immune response against o… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Immune response against ovalbumin in rats colonized with an ovalbumin-producing Escherichia coli and the influence of feeding ovalbumin.

Journal article
Authors Anna Dahlman-Höglund
Esbjörn Telemo
Staffan Ahlstedt
Lars Åke Hanson
Agnes E Wold
Ulf Dahlgren
Published in International archives of allergy and immunology
Volume 105
Issue 4
Pages 381-5
ISSN 1018-2438
Publication year 1994
Published at Institute of Laboratory Medicine, Dept of Clinical Immunology
Pages 381-5
Language en
Keywords Animals, Antibodies, Bacterial, biosynthesis, Escherichia coli, immunology, metabolism, Female, Freund's Adjuvant, Hypersensitivity, Delayed, immunology, Immunoglobulin E, biosynthesis, Intestines, immunology, microbiology, Ovalbumin, administration & dosage, biosynthesis, immunology, Rats, Rats, Sprague-Dawley
Subject categories Immunology


The influence of feeding ovalbumin (OA) on the development of IgE/IgG antibodies and delayed-type hypersensitivity (DTH) against OA was studied in rats colonized from birth with an Escherichia coli genetically manipulated to produce OA. At 21 days of age, colonized pups and pups with a normal intestinal flora were weaned onto either an OA-containing or a conventional diet without OA. At 2 months of age the colonized rats showed an increased DTH reaction to OA, but they did not have any anti-OA antibodies in serum. The rats were then immunized intracutaneously with OA in Freund's complete adjuvant. After immunization the colonized rats fed the conventional diet had a significantly higher DTH reaction to OA and significantly higher serum levels of IgE anti-OA antibodies than the uncolonized rats on the same diet. The colonized rats eating the OA-containing diet showed a 73% decrease in the DTH reaction to OA and also significantly lower levels of IgE and IgG antibodies against OA compared with the colonized rats fed conventional diet. The dams colonized as adults by the OA-producing E. coli developed IgE anti-lipopolysaccharide antibodies in serum while the pups colonized via the dams at birth did not. Neonatal colonization with an E. coli strain producing OA resulted in increased DTH reactivity against OA and priming for secondary IgE anti-OA response. Feeding the animals an OA-containing diet from weaning abrogated this intestinally induced hypersensitivity and rendered the animals orally tolerant to OA.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?

Denna text är utskriven från följande webbsida:
Utskriftsdatum: 2019-11-15