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Measuring the diffusion of fluorophores in human skin by two-photon fluorescence correlation spectroscopy combined with measurements of point spread function

Journal article
Authors Stina Guldbrand
Carl Simonsson
Mattias Goksör
Maria Smedh
Marica B Ericson
Published in MULTIPHOTON MICROSCOPY scigloo.IN THE BIOMEDICAL SCIENCES XI Book Series: Proceedings of SPIE
Volume 7903
Pages 7903291 - 7903296
Publication year 2011
Published at Department of Chemistry
Department of Physics (GU)
Pages 7903291 - 7903296
Language en
Keywords two photon, fluorescence correlation spectroscopy, point spread function, diffusion, human skin
Subject categories Physical Sciences, Optics

Abstract

Two-photon excitation fluorescence correlation spectroscopy (TPFCS) has been used in combination with measurements of the point spread function (PSF), for quantitative analysis of fluorophores in excised human skin. Measurements have been performed at depths between 0 and 40 μm. The PSF, measured as full width at half maximum, was found not to depend on the depth. Measurements revealed difference in diffusion coefficient depending on extra- or intracellular location of fluorophore. The number of molecules was accumulating close to the surface and then decreased by the depth. The results from our study show that TPFCS can be used for quantitative analyses of fluorescent compounds in human skin.

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Utskriftsdatum: 2019-11-17