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Distribution of cholesterol and galactosylceramide in rat cerebellar white matter.

Journal article
Authors Katrin Börner
Håkan Nygren
Birgit Hagenhoff
Per Malmberg
Elke Tallarek
Jan-Eric Månsson
Published in Biochimica et biophysica acta
Volume 1761
Issue 3
Pages 335-44
ISSN 0006-3002
Publication year 2006
Published at Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 335-44
Language en
Links dx.doi.org/10.1016/j.bbalip.2006.02...
Keywords Animals, Cerebellum, anatomy & histology, chemistry, metabolism, Cholesterol, chemistry, metabolism, Galactosylceramides, chemistry, metabolism, Male, Mass Spectrometry, Myelin Sheath, chemistry, Rats, Rats, Sprague-Dawley
Subject categories Medical and Health Sciences

Abstract

White matter and the inner granular layer of rat cerebellum was analysed by imaging time-of-flight secondary-ion mass spectrometry (TOF-SIMS) equipped with a Bi+ ion cluster gun. Samples were prepared by high pressure freezing, freeze-fracturing and freeze drying or by plunge freezing and cryostat sectioning. The identified and localized chemical species were: sodium, potassium, phosphocholine, cholesterol and galactosylceramide (GalC) with carbon chain lengths C18:0 (N-stearoyl-galactosylceramide) and C24:0 (N-lignoceroylgalactosylceramide) with CH24:0 (hydroxy-lignoceroylgalactosylceramide). We report new findings regarding the organization of myelin in white matter. One is cholesterol-rich, ribbon-shaped 10-20 microm areas excluding Na+ and K+. The second finding is the different distribution of GalC C18 and GalC C24 in relation to these areas, where GalC C18 was localized in cholesterol-rich areas and GalC C24 was localized in Na/K-enriched areas. The distribution of GalC was in small spots, homogeneous in size, of 0.8-1.5 microm. Sample preparation with high pressure freezing allowed separate localization of sodium and potassium in tissue samples.

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