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On-chip maturation of induced pluripotent stem-cell derived hepatocytes

Authors Philip Dalsbecker
Amin Abbaszadehbanaeiyan
Jannick Theobald
Stefan Wölfl
Caroline B. Adiels
Mattias Goksör
Published in SelectBio Organ-on-a-Chip Europe (conference), 10-11 May 2017, Munich, Germany.
Publication year 2017
Published at Department of Physics (GU)
Language en
Keywords Hepatocytes, liver on a chip, PDMS, microfluidics, bile canaliculi, induced pluripotent stem cells
Subject categories Biophysics, Cell Biology


The increasing interest in induced pluripotent stem cells (iPSCs) for research towards personalized medicine fits together well with recent advances in organ-on-chip development. Here, results of on-chip maturation of iPS-derived hepatocytes in a liver-on-a-chip platform are presented. The device itself, previously described by Banaeiyan et al., is designed to mimic the lobular structure of the liver and has successfully been used for 3D culture of both hepatocellular carcinoma cells (HepG2) and iPSC-derived hepatocytes. As the next step of device validation, iPS-derived hepatic cells have been matured on-chip into functional, mature hepatocytes, expressing important markers such as albumin and forming bile canalicular structures not seen in corresponding 2D cultures. Future plans for the platform include on-chip differentiation of iPSC-derived definitive endoderm cells into mature hepatocytes. To this end, a combination of immunofluorescent and metabolic assays have been tested on said definitive endoderm cells during directed differentiation into hepatocytes in a conventional 2D culture. These assays will be used to verify the corresponding differentiation in the on-chip 3D culture.

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