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Automated correction of linear deformation due to sectioning in serial micrographs.

Journal article
Authors T Jansson
T Gustavsson
M Rydmark
C H Berthold
R Pascher
Thomas Skoglund
Published in Journal of microscopy
Volume 177
Issue Pt 2
Pages 119-27
ISSN 0022-2720
Publication year 1995
Published at Institute of Anatomy and Cell Biology
Pages 119-27
Language en
Links www.ncbi.nlm.nih.gov/entrez/query.f...
Keywords Animals, Cats, Humans, Image Processing, Computer-Assisted, Microscopy, Microtomy
Subject categories Neuroscience

Abstract

This paper describes an objective and automatic method for detection and correction of sectioning deformations in digitized micrographs, as well as an evaluation of the method applied to light and electron microscopic images of semi-thin and ultra-thin serial sections from brain cortex. The detection is based on matching of image subregions and the deformation model is bi-linear, i.e. two first-order polynomials are used for modelling compression/expansion in perpendicular directions. The procedure is applicable to prealigned serial two-dimensional sections and is primarily aimed at three-dimensional reconstruction of tissue samples consisting of a large number of cells with random distribution and morphology.

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