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Sensitive liquid chromatography-electrospray mass spectrometry allows for the analysis of the O-glycosylation of immunoprecipitated proteins from cells or tissues: application to MUC1 glycosylation in cancer.

Journal article
Authors Malin Bäckström
Kristina A Thomsson
Hasse Karlsson
Gunnar C. Hansson
Published in Journal of proteome research
Volume 8
Issue 2
Pages 538-45
ISSN 1535-3893
Publication year 2009
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 538-45
Language en
Links dx.doi.org/10.1021/pr800713h
Keywords Carbohydrate Conformation, Carbohydrate Sequence, Cell Line, Chromatography, Liquid, methods, Glycosylation, Humans, Immunoprecipitation, Molecular Sequence Data, Mucin-1, chemistry, metabolism, Neoplasms, chemistry, diagnosis, metabolism, Sensitivity and Specificity, Spectrometry, Mass, Electrospray Ionization, methods
Subject categories Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Abstract

We have analyzed the structures of the glycans on immunoprecipitated proteins from small amounts of cell or tissue lysates, by liquid-chromatography electrospray mass spectrometry (LC-ESI-MS) and MS/MS. The sensitive and specific method was applied to the analysis of the O-glycosylation of MUC1 in breast, prostate and gastric cancer, including analysis of a patient tumor specimen. The method will be applicable for the glycosylation analysis of individual proteins.

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