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High-throughput and high-sensitivity nano-LC/MS and MS/MS for O-glycan profiling.

Journal article
Authors Hasse Karlsson
Jessica Holmén Larsson
Kristina A Thomsson
Iris van Dijk Härd
Malin Bäckström
Gunnar C. Hansson
Published in Methods in molecular biology (Clifton, N.J.)
Volume 534
Pages 117-31
ISSN 1064-3745
Publication year 2009
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 117-31
Language en
Links dx.doi.org/10.1007/978-1-59745-022-...
Keywords Blotting, Western, methods, Carbohydrate Sequence, Chromatography, Liquid, instrumentation, methods, Electrophoresis, Polyacrylamide Gel, methods, Glycomics, methods, Humans, Molecular Sequence Data, Mucins, analysis, chemistry, isolation & purification, Nanotechnology, instrumentation, methods, Polysaccharides, analysis, chemistry, isolation & purification, Sensitivity and Specificity, Tandem Mass Spectrometry, instrumentation, methods
Subject categories Chemistry, Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Abstract

Sensitive and fast methods for the profiling of biologically important molecules are highly demanded. Mucins are densely O-glycosylated glycoproteins found at mucosal surfaces and are of great medical interest. Here we describe sensitive methods for the analysis of O-glycans from mucins using gel electrophoresis, and chromatography by nanoLC on graphite columns and structural analysis by electrospray mass spectrometry on a linear trap mass spectrometer.

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Utskriftsdatum: 2019-11-18