To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Localization of cholester… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Localization of cholesterol, phosphocholine and galactosylceramide in rat cerebellar cortex with imaging TOF-SIMS equipped with a bismuth cluster ion source.

Journal article
Authors Håkan Nygren
Katrin Börner
Birgit Hagenhoff
Per Malmberg
Jan-Eric Månsson
Published in Biochimica et biophysica acta
Volume 1737
Issue 2-3
Pages 102-10
ISSN 0006-3002
Publication year 2005
Published at Institute of Anatomy and Cell Biology
Institute of Clinical Neurosciences, Section of Experimental Neuroscience
Pages 102-10
Language en
Keywords Animals, Bismuth, Cerebellar Cortex, metabolism, Cholesterol, metabolism, Galactosylceramides, metabolism, Male, Phosphorylcholine, metabolism, Purkinje Cells, metabolism, Rats, Rats, Sprague-Dawley, Spectrometry, Mass, Secondary Ion, methods, Tissue Distribution
Subject categories Medical and Health Sciences


Time-of-flight secondary-ion-mass-spectrometry (TOF-SIMS) was utilized to address the issue of co-localization of cholesterol, phosphocholine and galactosylceramide in rat cerebellar cortex. Rat cerebellum was fixed, freeze-protected by sucrose, frozen and sectioned by cryoultramicrotomy and dried at room temperature. The samples were analyzed in an imaging TOF-SIMS instrument equipped with a Bi(1-7)+-source. The cholesterol signal (m/z 369 and 385) was localized in Purkinje cells and in nuclei of granular layer cells. The phosphocholine headgroup of phosphatidylcholine and sphingomyelin was localized by imaging a specific fragment (m/z 86). This signal was localized in the molecular layer of cerebellar cortex, in Purkinje cells and in parts of the granular layer probably representing the synapse-rich glomeruli. The galactosylceramide was localized by imaging the quasi-molecular ions at m/z 835 and 851, showed a clear colocalization with cholesterol, but also a specific localization in dots (diameter

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?