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Detection of the CS20 colonization factor antigen in diffuse-adhering Escherichia coli strains.

Journal article
Authors Theresa J Ochoa
Fulton P Rivera
Maria Bernal
Rina Meza
Lucie Ecker
Ana I Gil
David Cepeda
Susan Mosquito
Erik Mercado
Ryan C Maves
Eric R Hall
Ann-Mari Svennerholm
Annette McVeigh
Stephen Savarino
Claudio F Lanata
Published in FEMS immunology and medical microbiology
Volume 60
Issue 2
Pages 186-9
ISSN 1574-695X
Publication year 2010
Published at Institute of Biomedicine, Department of Microbiology and Immunology
Pages 186-9
Language en
Keywords Antibodies, Monoclonal, Antigens, Bacterial, analysis, immunology, Antigens, Surface, analysis, immunology, Bacterial Adhesion, Child, Diarrhea, microbiology, Enteropathogenic Escherichia coli, genetics, immunology, metabolism, Enzyme-Linked Immunosorbent Assay, Escherichia coli, genetics, immunology, metabolism, pathogenicity, Escherichia coli Infections, microbiology, Escherichia coli Proteins, analysis, genetics, immunology, Fimbriae Proteins, analysis, Humans, Polymerase Chain Reaction, Shiga-Toxigenic Escherichia coli, genetics, immunology, metabolism
Subject categories Microbiology in the medical area


We analyzed a randomly selected group of 30 diffusely adherent (DAEC), 30 enteropathogenic, 30 enteroaggregative, and five Shiga toxin-producing Escherichia coli strains isolated from children with diarrhea. Enterotoxigenic E. coli (ETEC) colonization factors (CFs) were evaluated by a dot-blot assay using 21 CF-specific monoclonal antibodies. Out of 95 non-ETEC strains, three DAEC were found to express coli surface antigen 20 (CS20). No other E. coli expressed CFs. We confirmed the three CS20-positive strains as ETEC-negative by repeat PCR and as toxin-negative by ganglioside-GM1-enzyme-linked immunosorbent assay. To our knowledge, this is the first study that has identified currently recognized CFs in non-ETEC diarrheagenic E. coli strains identified using molecular methods. CFs may be an unrecognized relevant adherence factor in other E. coli, which may then play a role in pathogenesis and the immune response of the host.

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