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A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies

Journal article
Authors Amit Sharma
Peter Berntsen
Rajiv Harimoorthy
R. Appio
Jennie Sjöhamn
Michael Järvå
Alexander Björling
Greger Hammarin
Sebastian Westenhoff
Gisela Brändén
Richard Neutze
Published in Journal of Applied Crystallography
Volume 52
Pages 378-386
ISSN 0021-8898
Publication year 2019
Published at Department of Chemistry and Molecular Biology
Pages 378-386
Language en
Links dx.doi.org/10.1107/s160057671900190...
Keywords difference X-ray scattering, protein structural changes, structural dynamics, conformational-changes, photolysis, resolution
Subject categories Chemical Sciences

Abstract

The X-ray crystallography station I911-2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small- and wide-angle X-ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop, a sample stage and a sample delivery system. This setup incorporated external devices such as infrared lasers, LEDs and reaction mixers to induce conformational changes in macromolecules. This platform was evaluated through proof-of-principle experiments capturing light-induced conformational changes in phytochromes. A difference WAXS signature of conformational changes in a plant aquaporin was also demonstrated using caged calcium.

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