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Leading and lagging strand DNA synthesis in vitro by a reconstituted herpes simplex virus type 1 replisome.

Journal article
Authors Maria Falkenberg
I R Lehman
Per Elias
Published in Proceedings of the National Academy of Sciences of the United States of America
Volume 97
Issue 8
Pages 3896-900
ISSN 0027-8424
Publication year 2000
Published at Institute of Medical Biochemistry
Pages 3896-900
Language en
Keywords DNA, DNA Helicases, metabolism, DNA Primase, DNA Replication, genetics, DNA-Binding Proteins, Protein Binding, Simplexvirus, genetics, metabolism, Templates, Genetic, Viral Proteins, metabolism
Subject categories Virology, Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)


The synthesis of double-stranded DNA by a rolling circle mechanism was reconstituted in vitro with a replisome consisting of the DNA polymerase-UL42 complex and the heterotrimeric helicase-primase encoded by herpes simplex virus type 1. Okazaki fragments 3 kilobases in length and leading strands that may exceed 10 kilobases are produced. Lagging strand synthesis is stimulated by ribonucleoside triphosphates. DNA replication appears to be processive because it resists competition with an excess of (dT)(150)/(dA)(20). The single-strand DNA binding protein ICP8 is not required, and high concentrations of ICP8 can, in fact, inhibit lagging strand synthesis. The inhibition can, however, be overcome by the addition of an excess of the UL8 component of the helicase-primase. Rolling circle replication by the herpesvirus and bacteriophage T7 replisomes appears to proceed by a similar mechanism.

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