To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Allele Phasing Greatly Im… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Contact form








 


Note! If you want an answer on a question you must specify your email address




Allele Phasing Greatly Improves the Phylogenetic Utility of Ultraconserved Elements

Journal article
Authors Tobias Andermann
A. M. Fernandes
Urban Olsson
Mats H. Töpel
Bernard E. Pfeil
Bengt Oxelman
A. Aleixo
B. C. Faircloth
Alexandre Antonelli
Published in Systematic Biology
Volume 68
Issue 1
Pages 32-46
ISSN 1063-5157
Publication year 2019
Published at Department of Biological and Environmental Sciences
Pages 32-46
Language en
Links dx.doi.org/10.1093/sysbio/syy039
Keywords Aves, gene tree, heterozygous sites, mitochondrial genome, SNP, species tree, target enrichment, Trochilidae, divergence times, read alignment, target-capture, DNA-sequences, species, trees, gene trees, radiation, algorithms, markers, rivers, Evolutionary Biology
Subject categories Biological Sciences

Abstract

Advances in high-throughput sequencing techniques now allow relatively easy and affordable sequencing of large portions of the genome, even for nonmodel organisms. Many phylogenetic studies reduce costs by focusing their sequencing efforts on a selected set of targeted loci, commonly enriched using sequence capture. The advantage of this approach is that it recovers a consistent set of loci, each with high sequencing depth, which leads to more confidence in the assembly of target sequences. High sequencing depth can also be used to identify phylogenetically informative allelic variation within sequenced individuals, but allele sequences are infrequently assembled in phylogenetic studies. Instead, many scientists perform their phylogenetic analyses using contig sequences which result from the de novo assembly of sequencing reads into contigs containing only canonical nucleobases, and this may reduce both statistical power and phylogenetic accuracy. Here, we develop an easy-to-use pipeline to recover allele sequences from sequence capture data, and we use simulated and empirical data to demonstrate the utility of integrating these allele sequences to analyses performed under the multispecies coalescent model. Our empirical analyses of ultraconserved element locus data collected from the South American hummingbird genus Topaza demonstrate that phased allele sequences carry sufficient phylogenetic information to infer the genetic structure, lineage divergence, and biogeographic history of a genus that diversified during the last 3 myr. The phylogenetic results support the recognition of two species and suggest a high rate of gene flow across large distances of rainforest habitats but rare admixture across the Amazon River. Our simulations provide evidence that analyzing allele sequences leads to more accurate estimates of tree topology and divergence times than the more common approach of using contig sequences.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?

Denna text är utskriven från följande webbsida:
http://www.gu.se/english/research/publication/?feedbackForm=true&returnAddress=http%3A%2F%2Fwww.gu.se%2Fenglish%2Fresearch%2Fpublication%2F%3Fprint%3Dtrue%26publicationId%3D279433&recipientName=Webmaster&encodedEmail=YW5uaWthLmtvbGRlbml1c0BndS5zZQ&publicationId=279433
Utskriftsdatum: 2019-10-23