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DNA replication and DNA repair on a genome-wide scale at single nucleotide resolution level

Research group

Short description

Our research is focused on to understand how a stable genome is maintained from generation to generation. We study DNA replication and DNA repair by combining well established genetic and biochemical tools and techniques with Next-Gen sequencing.

Current projects in our group focus on the following:  

1. Genome-wide mapping of ribonucleotides in DNA. 

More than a million ribonucleotides are incorporated into the human genome per round of replication. We are interested in the causes and consequences of these ribonucleotides in our genome. We study where those ribonucleotides are incorporated in a number of model organisms including virus, yeast, mice and human. A number of pathways may be involved in removing these ribonucleotides. We analyze if ribonucleotides are differentially incorporated in various mutants that lack specific genes which codes for proteins that may be involved in the processing or removal of ribonucleotides in DNA.

2. Development of new methods to map genome-wide ribonucleotides in DNA  

Based on our recently developed HydEn-seq protocol to genome-wide map ribonucleotides in DNA, we continue to improve and develop tools to study where ribonucleotides are incorporated into our genome. We expect that a better understanding of how ribonucleotides in DNA contribute to genome stability and instability will ultimately impact biomedical science. 

Group members

Katrin Kreisel

Mahmood Faraz 

Andranik Durgaryan 

Alireza Nourizadeh 

Yuika Matsushita