Light Microscopy Techniques
The Centre for Cellular Imaging facility can offer advanced techniques that employs fluorescence to take the step into the sub-resolution domain for light microscopy.
Fluorescence Correlation Spectroscopy
Fluorescence Correlation Spectroscopy (FCS) is a technique for the study of molecular movements and interactions. In FCS a laser beam is focused and parked somewhere in the specimen, and the fluctuations in the fluorescence signal is monitored as a function of time.
Fluorescence Recovery After Photobleaching
Fluorescence Recovery After Photobleaching (FRAP) is used to measure the dynamics of 2D or 3D molecular mobility e.g. diffusion, transport or any other kind of movement of fluorescently labeled molecules in membranes or in living cells. In this technique, the recovery of fluorescence in a defined region of interest (ROI) of a sample after a bleaching event is monitored by taking a time series of images.
Laser Microdissection
Laser Microdissection and Pressure Catapulting (LMPC) technology from Carl Zeiss and developed by P.A.L.M. made non-contact sampling possible. The key function is the laser catapult: The specimen is microdissected by a focused laser beam. Then a defined laser pulse transports the cut piece of the specimen out of the object plane into a collection device.
- Cell Culture, non-contact Laser Capture Microdissection (LCM) and Recultivation (Extern länk)
- Live cells and molecular analysis (Extern länk)
- DNA Handling (Extern länk)
- RNA Handling (Extern länk)
- RNA extraction from frozen sections (Extern länk)
- RNA extraction from FFPE sections (Extern länk)
- Protein Handling (Extern länk)
- Immunofluorescence on frozen sections (Extern länk)
- Chromosome Preparation (Extern länk)