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Göteborgs universitet

Light Microscopy Techniques

The Centre for Cellular Imaging facility can offer advanced techniques that employs fluorescence to take the step into the sub-resolution domain for light microscopy.

Fluorescence Correlation Spectroscopy

Fluorescence Correlation Spectroscopy (FCS) is a technique for the study of molecular movements and interactions. In FCS a laser beam is focused and parked somewhere in the specimen, and the fluctuations in the fluorescence signal is monitored as a function of time.

Fluorescence Recovery After Photobleaching

Fluorescence Recovery After Photobleaching (FRAP) is used to measure the dynamics of 2D or 3D molecular mobility e.g. diffusion, transport or any other kind of movement of fluorescently labeled molecules in membranes or in living cells. In this technique, the recovery of fluorescence in a defined region of interest (ROI) of a sample after a bleaching event is monitored by taking a time series of images.

Fluorescence Resonance Energy Transfer

Fluorescence resonance/Förster energy transfer (FRET) is the radiationless transfer of energy between two molecules, which can occur if they are very close to each other (< 10 nm).

GFP Recognition After Bleaching (GRAB)

We have developed a simple correlative photo-oxidation method that allows for the direct ultrastructural visualization of the green fluorescence protein (GFP) upon illumination.