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Molecular Insights into Covalently Stained Carious Dentine Using Solid-State NMR and ToF-SIMS

Artikel i vetenskaplig tidskrift
Författare Ulrica S. Almhöjd
Peter Lingström
Åke Nilsson
Jörgen G Norén
S. Siljestrom
A. Ostlund
D. Bernin
Publicerad i Caries Research
Volym 51
Nummer/häfte 3
Sidor 255-263
ISSN 0008-6568
Publiceringsår 2017
Publicerad vid Institutionen för odontologi
Institutionen för kemi och molekylärbiologi
Institutionen för odontologi, sektion 3
Sidor 255-263
Språk English
Länkar https://doi.org/10.1159/000460828
Ämnesord Carious dentine, Covalent binding, Hydrazine, Normal dentine, NMR, ToF-SIMS, matrix proteins, peritubular dentin, cavity preparation, in-vitro, bone, c-13, spectroscopy, collagen, n-15, dye, Dentistry, Oral Surgery & Medicine
Ämneskategorier Kirurgisk forskning, Odontologi

Sammanfattning

Dyes currently used to stain carious dentine have a limited capacity to discriminate normal dentine from carious dentine, which may result in overexcavation. Consequently, finding a selective dye is still a challenge. However, there is evidence that hydrazine-based dyes, via covalent bonds to functional groups, bind specifically to carious dentine. The aim of this study was to investigate the possible formation of covalent bonds between carious dentine and N-15(2)-hydrazine and the hydrazine-based dye, N-15(2)-labelled Lucifer Yellow, respectively. Powdered dentine from extracted carious and normal teeth was exposed to the dyes, and the staining reactions were analysed using time-of-flight secondary ion mass spectrometry (ToF-SIMS), solid-state C-13-labelled nuclear magnetic resonance (NMR) and N-15-NMR spectroscopy. The results showed that N-15(2)-hydrazine and N-15(2)-labelled Lucifer Yellow both bind to carious dentine but not to normal dentine. It can thus be concluded that hydrazine-based dyes can be used to stain carious dentine and leave normal dentine unstained. (C) 2017 S. Karger AG, Basel

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