Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Anti-Inflammatory Effect of Titrated Extract of Centella asiatica in Phthalic Anhydride-Induced Allergic Dermatitis Animal Model

Artikel i vetenskaplig tidskrift
Författare J. H. Park
J. Y. Choi
D. J. Son
E. K. Park
M. J. Song
Mats Hellström
J. T. Hong
Publicerad i International Journal of Molecular Sciences
Volym 18
Nummer/häfte 4
ISSN 1422-0067
Publiceringsår 2017
Publicerad vid Institutionen för kliniska vetenskaper, sektionen för kirurgi och kirurgisk gastroforskning, Avdelningen för kirurgi
Språk English
Länkar doi.org/10.3390/ijms18040738
Ämnesord titrated extract of Centella asiatica, skin inflammation, atopic dermatitis, NF-kappa B, cytokine, IgE, nf-kappa-b, induced atopic-dermatitis, skin-lesions, mast-cells, topical, application, nc/nga mice, mouse model, inflammation, mechanisms, ige, Biochemistry & Molecular Biology, Chemistry
Ämneskategorier Klinisk medicin

Sammanfattning

Centella asiatica has potent antioxidant and anti-inflammatory properties. However, its anti-dermatitic effect has not yet been reported. In this study, we investigated the anti-dermatitic effects of titrated extract of Centella asiatica (TECA) in a phthalic anhydride (PA)-induced atopic dermatitis (AD) animal model as well as in vitro model. An AD-like lesion was induced by the topical application of five percent PA to the dorsal skin or ear of Hos: HR-1 mouse. After AD induction, 100 mu L of 0.2% and 0.4% of TECA (40 mu g or 80 mu g/cm(2)) was spread on the dorsum of the ear or back skin three times a week for four weeks. We evaluated dermatitis severity, histopathological changes and changes in protein expression byWestern blotting for inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and NF-kappa B activity, which were determined by electromobility shift assay (EMSA). We also measured TNF-alpha, IL-1 beta, IL-6, and IgE concentration in the blood of AD mice by enzyme-linked immunosorbent assay (ELISA). TECA treatment attenuated the development of PA-induced atopic dermatitis. Histological analysis showed that TECA inhibited hyperkeratosis, mast cells and infiltration of inflammatory cells. TECA treatment inhibited expression of iNOS and COX-2, and NF-kappa B activity as well as the release of TNF-alpha, IL-1 beta, IL-6, and IgE. In addition, TECA (1, 2, 5 mu g/mL) potently inhibited Lipopolysaccharide (LPS) (1 mu g/mL)-induced NO production, expression of iNOS and COX-2, and NF-kappa B DNA binding activities in RAW264.7 macrophage cells. Our data demonstrated that TECA could be a promising agent for AD by inhibition of NF-kappa B signaling.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?