Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Extracellular Osmotic Stress Reduces the Vesicle Size while Keeping a Constant Neurotransmitter Concentration

Artikel i vetenskaplig tidskrift
Författare H. Fathali
J. Dunevall
Soodabeh Majdi
A. S. Cans
Publicerad i Acs Chemical Neuroscience
Volym 8
Nummer/häfte 2
Sidor 368-375
ISSN 1948-7193
Publiceringsår 2017
Publicerad vid Institutionen för kemi och molekylärbiologi
Sidor 368-375
Språk English
Länkar doi.org/10.1021/acschemneuro.6b0035...
Ämnesord Osmotic stress, dense core vesicle, chromaffin cell, exocytosis, catecholamine concentration, vesicle, adrenal-medullary cells, bovine chromaffin cells, dense core vesicles, quantal size, intraneuronal disposition, electron-microscopy, secretory, granules, exocytotic release, vesicular volume, membrane-fusion, Biochemistry & Molecular Biology, Pharmacology & Pharmacy, Neurosciences, & Neurology
Ämneskategorier Neurovetenskaper, Farmakologi, Biokemi och molekylärbiologi

Sammanfattning

Secretory cells respond to hypertonic stress by cell shrinking, which causes a reduction in exocytosis activity and the amount of signaling molecules released from single exocytosis events. These changes in exocytosis have been suggested to result from alterations in biophysical properties of cell cytoplasm and plasma membrane, based on the assumption that osmotic stress does not affect the secretory vesicle content and size prior to exocytosis. To further investigate whether vesicles in secretory cells are affected by the osmolality of the extracellular environment, we used intracellular electrochemical cytometry together with transmission electron microscopy imaging to quantify and determine the catecholamine concentration of dense core vesicles in situ before and after cell exposure to osmotic stress. In addition, single cell amperometry recordings of exocytosis at chromaffin cells were used to monitor the effect on exocytosis activity and quantal release when cells were exposed to osmotic stress. Here we show that hypertonic stress hampers exocytosis secretion after the first pool of readily releasable vesicles have been fused and that extracellular osmotic stress causes catecholamine filled vesicles to shrink, mainly by reducing the volume of the halo solution surrounding the protein matrix in dense core vesicles. In addition, the vesicles demonstrate the ability to perform adjustments in neurotransmitter content during shrinking, and intracellular amperometry measurements in situ suggest that vesicles reduce the catecholamine content to maintain a constant concentration within the vesicle compartment. Hence, the secretory vesicles in the cell cytoplasm are highly affected and respond to extracellular osmotic stress, which gives a new perspective to the cause of reduction in quantal size by these vesicles when undergoing exocytosis.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?