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Role of microglia in a mouse model of paediatric traumatic brain injury.

Artikel i vetenskaplig tidskrift
Författare Vibol Chhor
Raffaella Moretti
Tifenn Le Charpentier
Stephanie Sigaut
Sophie Lebon
Leslie Schwendimann
Marie-Virginie Oré
Chiara Zuiani
Valentina Milan
Julien Josserand
Regina Vontell
Julien Pansiot
Vincent Degos
Chrysanthy Ikonomidou
Luigi Titomanlio
Henrik Hagberg
Pierre Gressens
Bobbi Fleiss
Publicerad i Brain, behavior, and immunity
Volym 63
Sidor 197–209
ISSN 1090-2139
Publiceringsår 2017
Publicerad vid Institutionen för kliniska vetenskaper, sektionen för kvinnors och barns hälsa, Avdelningen för obstetrik och gynekologi
Sidor 197–209
Språk en
Länkar dx.doi.org/10.1016/j.bbi.2016.11.00...
www.ncbi.nlm.nih.gov/entrez/query.f...
Ämneskategorier Experimentell hjärnforskning

Sammanfattning

The cognitive and behavioural deficits caused by traumatic brain injury (TBI) to the immature brain are more severe and persistent than TBI in the mature brain. Understanding this developmental sensitivity is critical as children under four years of age sustain TBI more frequently than any other age group. Microglia (MG), resident immune cells of the brain that mediate neuroinflammation, are activated following TBI in the immature brain. However, the type and temporal profile of this activation and the consequences of altering it are still largely unknown. In a mouse model of closed head weight drop paediatric brain trauma, we characterized i) the temporal course of total cortical neuroinflammation and the phenotype of ex vivo isolated CD11B-positive microglia/macrophage (MG/MΦ) using a battery of 32 markers, and ii) neuropathological outcome 1 and 5days post-injury. We also assessed the effects of targeting MG/MΦ activation directly, using minocycline a prototypical microglial activation antagonist, on these processes and outcome. TBI induced a moderate increase in both pro- and anti-inflammatory cytokines/chemokines in the ipsilateral hemisphere. Isolated cortical MG/MΦ expressed increased levels of markers of endogenous reparatory/regenerative and immunomodulatory phenotypes compared with shams. Blocking MG/MΦ activation with minocycline at the time of injury and 1 and 2days post-injury had only transient protective effects, reducing ventricular dilatation and cell death 1day post-injury but having no effect on injury severity at 5days. This study demonstrates that, unlike in adults, the role of MG/MΦ in injury mechanisms following TBI in the immature brain may not be negative. An improved understanding of MG/MΦ function in paediatric TBI could support translational efforts to design therapeutic interventions.

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