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Insulin released from titanium discs with insulin coatings- kinetics and biological activity

Artikel i vetenskaplig tidskrift
Författare Behnosh Öhrnell Malekzadeh
Maria Ransjö
Pentti Tengvall
Zivko Mladenović
Anna Westerlund
Publicerad i Journal of Biomedical Materials Research. Part B - Applied biomaterials
Volym 105
Nummer/häfte 7
Sidor 1847-1854
ISSN 1552-4973
Publiceringsår 2017
Publicerad vid Institutionen för odontologi
Institutionen för kliniska vetenskaper, sektionen för anestesi, biomaterial och ortopedi, Avdelningen för biomaterialvetenskap
Institutionen för odontologi, sektion 3
Sidor 1847-1854
Språk en
Länkar dx.doi.org/10.1002/jbm.b.33717
Ämnesord Insulin, biomaterial, surface modification, osteoblast, titanium
Ämneskategorier Hälsovetenskaper

Sammanfattning

Local administration of insulin from a titanium surface has been demonstrated to increase bone formation in non-diabetic rats. The authors hypothesised that insulin was released from the titanium surface and with preserved biological activity after the release. Thus, in the present in vitro study, human recombinant insulin was immobilized onto titanium discs, and the insulin release kinetics was evaluated using Electro-chemiluminescence immunoassay. Neutral Red uptake assay and a mineralization assay were used to evaluate the biological effects of insulin on human osteoblast-like MG-63 cells. The results confirmed that insulin was released from titanium surfaces during a six-week period. Furthermore, etching the disc prior to insulin coating, thickening of the insulin coating and incubation of the discs in serum-enriched cell culture medium increased the release. Another interesting finding was that longer storage time decreased the release of insulin. The released insulin had retained its biological activity, as demonstrated by the significant increase in cell number and a stimulated mineralization process, upon exposure to released insulin.

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