Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Crooked, coiled and crimpled are three Ly6-like proteins required for proper localization of septate junction components.

Artikel i vetenskaplig tidskrift
Författare Anna Nilton
Kenzi Oshima
Fariba Zare
Sunitha Byri
Ulf Nannmark
Kevin G Nyberg
Richard G Fehon
Anne Uv
Publicerad i Development (Cambridge, England)
Volym 137
Nummer/häfte 14
Sidor 2427-37
ISSN 1477-9129
Publiceringsår 2010
Publicerad vid Institutionen för biomedicin
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 2427-37
Språk en
Länkar dx.doi.org/10.1242/dev.052605
Ämnesord Animals, Cell Membrane, genetics, metabolism, Cytoplasm, genetics, metabolism, Drosophila, genetics, metabolism, Intercellular Junctions, genetics, metabolism, Physiological Processes, genetics, Protein Binding, genetics, Proteins, genetics, metabolism, Tight Junctions, genetics, metabolism
Ämneskategorier Cell- och molekylärbiologi

Sammanfattning

Cellular junction formation is an elaborate process that is dependent on the regulated synthesis, assembly and membrane targeting of constituting components. Here, we report on three Drosophila Ly6-like proteins essential for septate junction (SJ) formation. SJs provide a paracellular diffusion barrier and appear molecularly and structurally similar to vertebrate paranodal septate junctions. We show that Crooked (Crok), a small GPI-anchored Ly6-like protein, is required for septa formation and barrier functions. In embryos that lack Crok, SJ components are produced but fail to accumulate at the plasma membrane. Crok is detected in intracellular puncta and acts tissue-autonomously, which suggests that it resides in intracellular vesicles to assist the cell surface localization of SJ components. In addition, we demonstrate that two related Ly6 proteins, Coiled (Cold) and Crimpled (Crim), are required for SJ formation and function in a tissue-autonomous manner, and that Cold also localizes to intracellular vesicles. Specifically, Crok and Cold are required for correct membrane trafficking of Neurexin IV, a central SJ component. The non-redundant requirement for Crok, Cold, Crim and Boudin (Bou; another Ly6 protein that was recently shown to be involved in SJ formation) suggests that members of this conserved family of proteins cooperate in the assembly of SJ components, possibly by promoting core SJ complex formation in intracellular compartments associated with membrane trafficking.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?