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Early indications on UVC-related carcinogenesis: premutagenic DNA damage in mice following 222-nm light exposure

Poster (konferens)
Författare Sofia Saadati
Manuela Buonanno
Gerhard Randers-Pehrson
Nils Rudqvist
Eva Forssell-Aronsson
David J. Brenner
Publicerad i 4th Swedish Cancer Research Meeting, Gothenburg, Sweden, November 7-8, 2016
Publiceringsår 2016
Publicerad vid Institutionen för kliniska vetenskaper, sektionen för onkologi, radiofysik, radiologi och urologi, Avdelningen för radiofysik
Språk en
Ämneskategorier Radiofysik, Klinisk medicin, Fysik

Sammanfattning

Surgical site infections, caused by air borne bacteria and viruses, can have lethal consequences. The use of conventional germicidal UV lamps, emitting primarily 254-nm light, at the surgical site has been prevented by being cancerogenic. Far-UVC light (200-222 nm) has been shown to be equieffective without being as damaging to human cells in vitro. In mammalian skin, it is DNA-damage in the basal cells that contributes to the major risk of cancer induction. The aim was to test the hypothesis that 222-nm light is less premutagenic than 254-nm light in vivo. SKH1 mice were exposed to different doses of 222-nm light and compared with positive (254-nm light) and negative (sham irradiated) controls. The mice were killed after 48h and dorsal skin samples were excised. UV specific DNA-damage cyclobutane pyrimidine dimer (CPD) was assessed by immunohistochemical analysis and a damage depth profile was determined. Loss of light intensity was also determined using phantoms with different protein concentrations, simulating various depths in skin. While the amount of CPD was significantly lower following 222-nm light in comparison with 254-nm light at all epidermal depths, no difference was seen in comparison with negative controls. Absorption of 222-nm light was much higher than 254-nm light. Results show that 222-nm light, for the investigated doses, does not appear to be premutagenic. These promising results indicate the potentials of far-UVC lamps as a disinfecting tool without patient and staff being subject to hazardous exposure.

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