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Eosinophils from eosinophilic oesophagitis patients have T cell suppressive capacity and express FOXP3.

Journal article
Authors Christine Lingblom
Jennie Andersson
Madeleine Ingelsten
Henrik Bergquist
Mogens Bove
Robert Saalman
Amanda Welin
Christine Wennerås
Published in Clinical and Experimental Immunology
Volume 187
Issue 3
Pages 455-465
ISSN 1365-2249
Publication year 2017
Published at Institute of Medicine, Department of Rheumatology and Inflammation Research
Institute of Clinical Sciences, Section for Anesthesiology, Biomaterials and Orthopaedics, Department of Otorhinolaryngology
Institute of Biomedicine, Department of Infectious Medicine
Institute of Clinical Sciences, Section for the Health of Women and Children, Department of Pediatrics
Pages 455-465
Language en
Links dx.doi.org/10.1111/cei.12898
www.ncbi.nlm.nih.gov/entrez/query.f...
Subject categories Allergology, Gastroenterology and Hepatology, Otorhinolaryngology

Abstract

Eosinophilic esophagitis (EoE) is an antigen-driven T cell-mediated chronic inflammatory disease where food and environmental antigens are thought to have a role. Human eosinophils express the immunoregulatory protein galectin-10 and have T cell suppressive capacity similar to regulatory T cells (Tregs ). We hypothesized that one function of eosinophils in EoE might be to regulate the T cell-driven inflammation in the oesophagus. This was tested by evaluating the suppressive capacity of eosinophils isolated from the blood of adult EoE patients in a mixed lymphocyte reaction. In addition, eosinophilic expression of forkhead box protein 3 (FOXP3), the canonical transcription factor of Tregs , was determined by conventional and imaging flow cytometry, quantitative polymerase chain reaction (qPCR), confocal microscopy and immunoblotting. It was found that blood eosinophils from EoE patients had T cell suppressive capacity, and that a fraction of the eosinophils expressed FOXP3. A comparison of EoE eosinophils with healthy control eosinophils indicated that the patients' eosinophils had inferior suppressive capacity. Furthermore, a higher percentage of the EoE eosinophils expressed FOXP3 protein compared with the healthy eosinophils, and they also had higher FOXP3 protein and mRNA levels. FOXP3 was found in the cytosol and nucleus of the eosinophils from both the patients and healthy individuals, contrasting with the strict nuclear localization of FOXP3 in Tregs . To conclude, these findings suggest that the immunoregulatory function of eosinophils may be impaired in EoE.

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